New method developed at UofL enables more extensive preclinical testing of heart drugs and therapies Researchers extend life of heart segments from 24 hours to six days; process allows better testing for efficacy and toxicity and saves money and time

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    Heart slices in the physiological biomimetic culture system
    Heart slices in the physiological biomimetic culture system developed at UofL

    LOUISVILLE, Ky. – Researchers at the University of Louisville have developed an easily reproducible system that enables them to keep slices of human hearts alive for a longer period of time, allowing more extensive testing of new drugs and gene therapies.

    This new biomimetic culture system mimics the environment of a living organ through continuous electrical stimulation and oxygenation, maintaining viability and functionality of the heart segments for six days. Previous culture systems maintained functional heart slices for no more than 24 hours. The extended viability time will enable improved preclinical testing of new drugs for effectiveness and toxicity.

    “This new method maintains fully functional human heart slices for six days in the culture environment. This facilitates testing efficacy of heart failure therapeutics and cardiotoxins on human heart tissue with no need for a living human,” said Tamer M. A. Mohamed, Ph.D., who led the research.

    The system provides access to the complete 3D multicellular system that reflects the heart’s functional and structural condition in a living person.

    “This system will save time and costs of clinical trials during phase one research, which includes testing for toxicity and proof of efficacy,” Mohamed said. “In addition to drugs, we have demonstrated the system’s effectiveness in testing gene therapy.”

    The optimized medium for sustaining the heart tissue slices is described in an article published online last week in Circulation Research, a publication of the American Heart Association. It will appear in the August 30, 2019, print and online issue. Along with Mohamed, the research was conducted by Qinghui Ou, B.Sc., Riham R.E. Abouleisa, Ph.D., and others at UofL, along with colleagues in California, Colorado, the United Kingdom, Germany and Egypt. UofL has a provisional patent application on this technology.

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    Tamer Mohamed, Ph.D.
    Tamer Mohamed, Ph.D.
    Qinghui Ou, B.Sc.
    Qinghui Ou, B.Sc.
    Riham Abouleisa, Ph.D.
    Riham Abouleisa, Ph.D.

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    Betty Coffman
    Betty Coffman is a Health Communications Specialist, working on the Health Sciences Campus with departments in the School of Medicine. A UofL alumna and Louisville native, she served as a writer and editor for local and national publications and as an account services coordinator and copywriter for marketing and design firms prior to joining UofL’s Office of Marketing and Communications.